FastQCFastQC Report
Wed 27 Apr 2022
G192M37_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG192M37_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17632450
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTTACCACGATCATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC824530.4676207787346625Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATTGTC251660.14272548624836592No Hit
CAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAAATT184430.10459692215205488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)