FastQCFastQC Report
Tue 7 May 2024
KC14_101_WT_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameKC14_101_WT_2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43228220
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT698470.1615773214811991No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT676650.15652969287192486No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG676080.1563978345627No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT525340.12152709503190277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)