FastQCFastQC Report
Mon 18 Apr 2022
SRR6723883_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6723883_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5247878
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCC514620.9806249306862698No Hit
CTGTCGACCGTGCGCTTCTTCTAGCCCAGTGATCAGTCATGGCATGCCCT302210.5758708567539108No Hit
ATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCCTATTCCCAT145780.2777884699301318No Hit
TTTGCTGTTCGTGACATGAGGCAGACAGTTGCTGTGGGTGTCATCAAAGC144310.2749873377391776No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC136560.260219463943331TruSeq Adapter, Index 1 (100% over 50bp)
TGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCAT104970.20002370481935747No Hit
TCGAGTTTCAGAACGATCGCGGGGGCCGTGCACTCTTCCAGGATGTGCAG103330.19689863217094605No Hit
TGATGGATGATCTGGACCGTAACAAGGATCAGGAAGTAAACTTCCAGGAG99100.18883823137656783No Hit
ATAATTGCTCTCCCCTCTCTACGCATTCTATATATAATAGACGAAATCAA82130.15650135159392045No Hit
AATAAGCACAACCGGCTGTACATGAAGGCCAGGCCCTTCCCTGATGGCCT66510.12673694014990441No Hit
TTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGG63480.12096317787875403No Hit
AGAGGCACCATTGAAATTCTGAGTGATGTGCAGCTGATAAAGACTGGAGA62940.11993419054330151No Hit
ATTGTCACACAGCCCACATAGCATGCAAGTTTGCTGAGCTTAAAGAAAAG61180.11658045404256731No Hit
TTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCA61170.11656139872154041No Hit
TTTACTGTGTGAACTCTGACAATAGCGGCTGGACCGAAATCCGTCGGGAA55610.10596664023058464No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)