FastQCFastQC Report
Mon 18 Apr 2022
SRR6723883_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6723883_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5247878
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT133450.25429325910396544Illumina RNA PCR Primer (100% over 50bp)
TGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCG109770.20917025891226895No Hit
GGAAGGCGACATACTCCTGGAAGTTTACTTCCTGATCCTTGTTACGGTCC108250.20627385011618027No Hit
GGCCATAGAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGT105400.20084308362351413No Hit
CATTGTAGATCAAAGCCAAGGCCCCCAGGAAGGCGACATACTCCTGGAAG96430.18375046066238584No Hit
GGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTCGG75890.14461083127313554No Hit
CCTTGCCAGAGTACTTGTGGAAGATGGCCACGAGAAGGCCAATGGCCTGA72030.1372554773567526No Hit
GCCAGCTCCAGCAGCCTTCTTGTCCACAGCTTTGATGACACCCACAGCAA71080.1354452218591972No Hit
GGTGTGAGGTCAGGCCACCAAGTCCACAGAGGGTGATTGCCGGTTATTGC62190.1185050414662841No Hit
GAGATGGCTTCTGCACATCCTGGAAGAGTGCACGGCCCCCGCGATCGTTC57170.10893927031078085No Hit
CCTGATCCTTGTTACGGTCCAGATCATCCATCAGCCTTGCAATTTCAGCA55260.10529970399464318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)