FastQCFastQC Report
Mon 18 Apr 2022
SRR7181426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7181426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4985294
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG279610.5608696297550355No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA200930.4030454372400103No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG129080.25892154003354667No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC126530.25380649566505004No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA123030.2467858465318194No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC93770.1880932197780111No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG74440.14931917756505433No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT73550.1475339267854614No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA69300.13900885283796705No Hit
GGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAG68310.1370230120831389No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC65050.13048377889047266No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT63260.12689321833376327TruSeq Adapter, Index 27 (97% over 39bp)
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG62330.12502773156407626No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT61860.12408495868047101No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC60210.12077522408909085No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC59830.12001298218319721No Hit
GGGAACGACGCGCGGGTGGAGGGGTCGGGAGGAACGGGGAGCGGGAAAGA58400.1171445455373344No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC57180.11469734783946543No Hit
GTCGGGAGGAACGGGGAGCGGGAAAGATCCGCCGGGACCACCGGCACGGC54140.10859941259231652No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA53200.106713866825106No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA51220.10274218531544979No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC51130.10256165433773816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)