FastQCFastQC Report
Mon 18 Apr 2022
SRR7181431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7181431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5022404
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG280160.5578205178237354No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA207020.412193045402162No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG134610.2680190601950779No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC126380.25163248516049286No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA124150.2471923803819844No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC96490.1921191525014714No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT77170.15365151827690485No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG74540.1484149821479913No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA70330.14003254218497757No Hit
GGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAG69390.13816092851152556No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC66340.13208813946468662No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT65460.1303359904937954TruSeq Adapter, Index 27 (97% over 39bp)
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT63880.12719008665969525No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC63310.12605517198536798No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCG61420.12229203385470384No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC60000.11946470256076572No Hit
GGGAACGACGCGCGGGTGGAGGGGTCGGGAGGAACGGGGAGCGGGAAAGA58840.11715505164459092No Hit
GTGCGCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACC55890.11128137043535327No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA54790.10909118422173923No Hit
GTCGGGAGGAACGGGGAGCGGGAAAGATCCGCCGGGACCACCGGCACGGC52680.1048900088483523No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC52530.10459134709195038No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA52090.10371527260650476No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)