FastQCFastQC Report
Mon 18 Apr 2022
SRR7181433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7181433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4978374
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA194320.39032824773711255No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC145670.2926055776444277No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG124680.2504432170021778No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC120390.24182594557982184No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC112990.2269616545482521No Hit
GTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGAT111210.2233861899487664No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC89890.18056096227402763No Hit
GCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC89840.18046052787516567No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG82820.16635953827494682No Hit
GCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTT76780.1542270628924223No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG76630.15392575969583644No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT73300.14723682873163005No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG70970.14255658574466282No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT66430.13343714232799705Illumina Single End PCR Primer 1 (97% over 34bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC61380.12329326804293932No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA59370.11925580520868863No Hit
CAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG54290.10905167028431371No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT54090.10864993268886589No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG52950.10636002839481325No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)