FastQCFastQC Report
Mon 18 Apr 2022
SRR7181477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR7181477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4595704
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC197610.42998852841697377No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC166740.36281710049211174No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC138620.3016295218316933No Hit
GGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGA106090.23084602489629444No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC101250.22031445019087392No Hit
GGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCG88300.19213596001831273No Hit
GTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGAT79440.17285708566086938No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG72420.15758195044763543No Hit
CAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG66490.1446785954883082No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG66160.14396053357657498No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT63290.13771557088968306No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC57670.12548675893834765No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG52540.1143241601286767No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG52300.1138019332837798No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT50850.1106468127625278No Hit
GCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGC48520.10557686047665384No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA48090.10464120404621359No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC47570.103509712548937No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC46950.1021606265329534No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC46450.10107265393941821No Hit
CAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCT46010.10011523805710724No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)