A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
Report generated on 2024-07-23, 12:59 based on data in:
/projectnb/wax-dk/max/G174_G178_2024/G174/SAMPLES
/projectnb/wax-dk/max/G174_G178_2024/G174/Scripts/03_FASTQC
/projectnb/wax-dk/max/G174_G178_2024/G174/Scripts/06_CollectMetrics
General Statistics
Showing 65/65 rows and 5/6 columns.Sample Name | M Reads Mapped | % Assigned | M Assigned | % Aligned | M Aligned |
---|---|---|---|---|---|
G174_M10_G174_M10 | 79.0% | 21.2 | |||
G174_M10_sorted | 94.4% | 20.0 | |||
G174_M10_statistics_for_all_accepted_reads | 49.4 | ||||
G174_M10_statistics_for_primary_reads | 45.0 | ||||
G174_M10_statistics_for_primary_unique_reads | 42.4 | ||||
G174_M11_G174_M11 | 80.4% | 26.2 | |||
G174_M11_sorted | 94.7% | 24.8 | |||
G174_M11_statistics_for_all_accepted_reads | 60.7 | ||||
G174_M11_statistics_for_primary_reads | 55.5 | ||||
G174_M11_statistics_for_primary_unique_reads | 52.3 | ||||
G174_M12_G174_M12 | 80.9% | 19.2 | |||
G174_M12_sorted | 95.3% | 18.3 | |||
G174_M12_statistics_for_all_accepted_reads | 44.5 | ||||
G174_M12_statistics_for_primary_reads | 40.6 | ||||
G174_M12_statistics_for_primary_unique_reads | 38.4 | ||||
G174_M13_G174_M13 | 73.6% | 17.7 | |||
G174_M13_sorted | 94.0% | 16.6 | |||
G174_M13_statistics_for_all_accepted_reads | 42.2 | ||||
G174_M13_statistics_for_primary_reads | 37.8 | ||||
G174_M13_statistics_for_primary_unique_reads | 35.4 | ||||
G174_M1_G174_M1 | 62.4% | 12.7 | |||
G174_M1_sorted | 94.1% | 12.0 | |||
G174_M1_statistics_for_all_accepted_reads | 31.6 | ||||
G174_M1_statistics_for_primary_reads | 27.4 | ||||
G174_M1_statistics_for_primary_unique_reads | 25.4 | ||||
G174_M2_G174_M2 | 76.8% | 21.3 | |||
G174_M2_sorted | 95.5% | 20.3 | |||
G174_M2_statistics_for_all_accepted_reads | 51.1 | ||||
G174_M2_statistics_for_primary_reads | 45.4 | ||||
G174_M2_statistics_for_primary_unique_reads | 42.6 | ||||
G174_M3_G174_M3 | 79.7% | 20.1 | |||
G174_M3_sorted | 95.5% | 19.2 | |||
G174_M3_statistics_for_all_accepted_reads | 47.6 | ||||
G174_M3_statistics_for_primary_reads | 42.7 | ||||
G174_M3_statistics_for_primary_unique_reads | 40.2 | ||||
G174_M4_G174_M4 | 64.3% | 10.7 | |||
G174_M4_sorted | 93.5% | 10.0 | |||
G174_M4_statistics_for_all_accepted_reads | 27.3 | ||||
G174_M4_statistics_for_primary_reads | 23.3 | ||||
G174_M4_statistics_for_primary_unique_reads | 21.5 | ||||
G174_M5_G174_M5 | 76.4% | 24.9 | |||
G174_M5_sorted | 94.9% | 23.6 | |||
G174_M5_statistics_for_all_accepted_reads | 59.9 | ||||
G174_M5_statistics_for_primary_reads | 53.2 | ||||
G174_M5_statistics_for_primary_unique_reads | 49.7 | ||||
G174_M6_G174_M6 | 69.5% | 15.6 | |||
G174_M6_sorted | 94.3% | 14.7 | |||
G174_M6_statistics_for_all_accepted_reads | 38.4 | ||||
G174_M6_statistics_for_primary_reads | 33.6 | ||||
G174_M6_statistics_for_primary_unique_reads | 31.1 | ||||
G174_M7_G174_M7 | 77.8% | 23.4 | |||
G174_M7_sorted | 95.3% | 22.3 | |||
G174_M7_statistics_for_all_accepted_reads | 54.1 | ||||
G174_M7_statistics_for_primary_reads | 49.5 | ||||
G174_M7_statistics_for_primary_unique_reads | 46.8 | ||||
G174_M8_G174_M8 | 80.2% | 23.4 | |||
G174_M8_sorted | 95.1% | 22.3 | |||
G174_M8_statistics_for_all_accepted_reads | 53.5 | ||||
G174_M8_statistics_for_primary_reads | 49.3 | ||||
G174_M8_statistics_for_primary_unique_reads | 46.8 | ||||
G174_M9_G174_M9 | 72.3% | 10.4 | |||
G174_M9_sorted | 93.6% | 9.8 | |||
G174_M9_statistics_for_all_accepted_reads | 26.1 | ||||
G174_M9_statistics_for_primary_reads | 22.8 | ||||
G174_M9_statistics_for_primary_unique_reads | 20.9 |
RSeQC
RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.
Infer experiment
Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).
featureCounts
Subread featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations.
Samtools
Samtools is a suite of programs for interacting with high-throughput sequencing data.
Samtools Flagstat
This module parses the output from samtools flagstat
. All numbers in millions.