A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
Report generated on 2024-07-23, 12:49 based on data in:
/projectnb/wax-dk/max/G174_G178_2024/G175/SAMPLES
/projectnb/wax-dk/max/G174_G178_2024/G175/Scripts/03_FASTQC
/projectnb/wax-dk/max/G174_G178_2024/G175/Scripts/06_CollectMetrics
General Statistics
Showing 65/65 rows and 5/6 columns.Sample Name | M Reads Mapped | % Assigned | M Assigned | % Aligned | M Aligned |
---|---|---|---|---|---|
G175_M10_G175_M10 | 83.7% | 13.2 | |||
G175_M10_sorted | 95.9% | 12.7 | |||
G175_M10_statistics_for_all_accepted_reads | 29.4 | ||||
G175_M10_statistics_for_primary_reads | 27.5 | ||||
G175_M10_statistics_for_primary_unique_reads | 26.4 | ||||
G175_M11_G175_M11 | 80.6% | 12.3 | |||
G175_M11_sorted | 95.2% | 11.7 | |||
G175_M11_statistics_for_all_accepted_reads | 27.8 | ||||
G175_M11_statistics_for_primary_reads | 25.7 | ||||
G175_M11_statistics_for_primary_unique_reads | 24.6 | ||||
G175_M12_G175_M12 | 82.8% | 15.4 | |||
G175_M12_sorted | 95.1% | 14.6 | |||
G175_M12_statistics_for_all_accepted_reads | 34.9 | ||||
G175_M12_statistics_for_primary_reads | 32.2 | ||||
G175_M12_statistics_for_primary_unique_reads | 30.7 | ||||
G175_M13_G175_M13 | 80.9% | 10.3 | |||
G175_M13_sorted | 94.9% | 9.8 | |||
G175_M13_statistics_for_all_accepted_reads | 23.4 | ||||
G175_M13_statistics_for_primary_reads | 21.6 | ||||
G175_M13_statistics_for_primary_unique_reads | 20.6 | ||||
G175_M1_G175_M1 | 72.4% | 15.1 | |||
G175_M1_sorted | 94.8% | 14.3 | |||
G175_M1_statistics_for_all_accepted_reads | 35.3 | ||||
G175_M1_statistics_for_primary_reads | 31.7 | ||||
G175_M1_statistics_for_primary_unique_reads | 30.1 | ||||
G175_M2_G175_M2 | 73.5% | 13.8 | |||
G175_M2_sorted | 95.0% | 13.1 | |||
G175_M2_statistics_for_all_accepted_reads | 32.1 | ||||
G175_M2_statistics_for_primary_reads | 29.0 | ||||
G175_M2_statistics_for_primary_unique_reads | 27.5 | ||||
G175_M3_G175_M3 | 77.1% | 19.4 | |||
G175_M3_sorted | 94.0% | 18.2 | |||
G175_M3_statistics_for_all_accepted_reads | 44.3 | ||||
G175_M3_statistics_for_primary_reads | 40.6 | ||||
G175_M3_statistics_for_primary_unique_reads | 38.8 | ||||
G175_M4_G175_M4 | 73.0% | 15.5 | |||
G175_M4_sorted | 95.4% | 14.8 | |||
G175_M4_statistics_for_all_accepted_reads | 37.0 | ||||
G175_M4_statistics_for_primary_reads | 32.9 | ||||
G175_M4_statistics_for_primary_unique_reads | 31.0 | ||||
G175_M5_G175_M5 | 78.3% | 14.0 | |||
G175_M5_sorted | 94.8% | 13.3 | |||
G175_M5_statistics_for_all_accepted_reads | 32.4 | ||||
G175_M5_statistics_for_primary_reads | 29.4 | ||||
G175_M5_statistics_for_primary_unique_reads | 28.0 | ||||
G175_M6_G175_M6 | 77.3% | 14.0 | |||
G175_M6_sorted | 95.3% | 13.3 | |||
G175_M6_statistics_for_all_accepted_reads | 32.4 | ||||
G175_M6_statistics_for_primary_reads | 29.4 | ||||
G175_M6_statistics_for_primary_unique_reads | 27.9 | ||||
G175_M7_G175_M7 | 77.8% | 14.8 | |||
G175_M7_sorted | 95.8% | 14.1 | |||
G175_M7_statistics_for_all_accepted_reads | 32.9 | ||||
G175_M7_statistics_for_primary_reads | 30.7 | ||||
G175_M7_statistics_for_primary_unique_reads | 29.5 | ||||
G175_M8_G175_M8 | 81.1% | 14.6 | |||
G175_M8_sorted | 95.7% | 14.0 | |||
G175_M8_statistics_for_all_accepted_reads | 32.5 | ||||
G175_M8_statistics_for_primary_reads | 30.4 | ||||
G175_M8_statistics_for_primary_unique_reads | 29.3 | ||||
G175_M9_G175_M9 | 74.6% | 14.0 | |||
G175_M9_sorted | 95.4% | 13.4 | |||
G175_M9_statistics_for_all_accepted_reads | 31.7 | ||||
G175_M9_statistics_for_primary_reads | 29.2 | ||||
G175_M9_statistics_for_primary_unique_reads | 28.0 |
RSeQC
RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.
Infer experiment
Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).
featureCounts
Subread featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations.
Samtools
Samtools is a suite of programs for interacting with high-throughput sequencing data.
Samtools Flagstat
This module parses the output from samtools flagstat
. All numbers in millions.