A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
Report generated on 2024-07-23, 12:55 based on data in:
/projectnb/wax-dk/max/G174_G178_2024/G176/SAMPLES
/projectnb/wax-dk/max/G174_G178_2024/G176/Scripts/03_FASTQC
/projectnb/wax-dk/max/G174_G178_2024/G176/Scripts/06_CollectMetrics
General Statistics
Showing 65/65 rows and 5/6 columns.Sample Name | M Reads Mapped | % Assigned | M Assigned | % Aligned | M Aligned |
---|---|---|---|---|---|
G176_M10_G176_M10 | 77.6% | 12.7 | |||
G176_M10_sorted | 94.6% | 12.1 | |||
G176_M10_statistics_for_all_accepted_reads | 29.1 | ||||
G176_M10_statistics_for_primary_reads | 26.8 | ||||
G176_M10_statistics_for_primary_unique_reads | 25.5 | ||||
G176_M11_G176_M11 | 79.1% | 15.2 | |||
G176_M11_sorted | 94.1% | 14.3 | |||
G176_M11_statistics_for_all_accepted_reads | 34.7 | ||||
G176_M11_statistics_for_primary_reads | 32.1 | ||||
G176_M11_statistics_for_primary_unique_reads | 30.4 | ||||
G176_M12_G176_M12 | 80.3% | 14.9 | |||
G176_M12_sorted | 95.7% | 14.3 | |||
G176_M12_statistics_for_all_accepted_reads | 33.6 | ||||
G176_M12_statistics_for_primary_reads | 31.2 | ||||
G176_M12_statistics_for_primary_unique_reads | 29.8 | ||||
G176_M13_G176_M13 | 80.3% | 13.7 | |||
G176_M13_sorted | 95.2% | 13.0 | |||
G176_M13_statistics_for_all_accepted_reads | 30.7 | ||||
G176_M13_statistics_for_primary_reads | 28.5 | ||||
G176_M13_statistics_for_primary_unique_reads | 27.3 | ||||
G176_M1_G176_M1 | 71.5% | 14.1 | |||
G176_M1_sorted | 95.3% | 13.5 | |||
G176_M1_statistics_for_all_accepted_reads | 33.2 | ||||
G176_M1_statistics_for_primary_reads | 29.8 | ||||
G176_M1_statistics_for_primary_unique_reads | 28.2 | ||||
G176_M2_G176_M2 | 74.2% | 15.3 | |||
G176_M2_sorted | 95.6% | 14.6 | |||
G176_M2_statistics_for_all_accepted_reads | 35.8 | ||||
G176_M2_statistics_for_primary_reads | 32.3 | ||||
G176_M2_statistics_for_primary_unique_reads | 30.6 | ||||
G176_M3_G176_M3 | 76.2% | 11.7 | |||
G176_M3_sorted | 95.6% | 11.2 | |||
G176_M3_statistics_for_all_accepted_reads | 26.7 | ||||
G176_M3_statistics_for_primary_reads | 24.6 | ||||
G176_M3_statistics_for_primary_unique_reads | 23.4 | ||||
G176_M4_G176_M4 | 75.1% | 15.1 | |||
G176_M4_sorted | 96.2% | 14.5 | |||
G176_M4_statistics_for_all_accepted_reads | 35.6 | ||||
G176_M4_statistics_for_primary_reads | 31.9 | ||||
G176_M4_statistics_for_primary_unique_reads | 30.2 | ||||
G176_M5_G176_M5 | 78.2% | 21.1 | |||
G176_M5_sorted | 96.1% | 20.3 | |||
G176_M5_statistics_for_all_accepted_reads | 49.2 | ||||
G176_M5_statistics_for_primary_reads | 44.6 | ||||
G176_M5_statistics_for_primary_unique_reads | 42.3 | ||||
G176_M6_G176_M6 | 77.6% | 11.7 | |||
G176_M6_sorted | 95.8% | 11.2 | |||
G176_M6_statistics_for_all_accepted_reads | 27.1 | ||||
G176_M6_statistics_for_primary_reads | 24.7 | ||||
G176_M6_statistics_for_primary_unique_reads | 23.5 | ||||
G176_M7_G176_M7 | 78.8% | 21.8 | |||
G176_M7_sorted | 95.6% | 20.8 | |||
G176_M7_statistics_for_all_accepted_reads | 47.6 | ||||
G176_M7_statistics_for_primary_reads | 45.0 | ||||
G176_M7_statistics_for_primary_unique_reads | 43.5 | ||||
G176_M8_G176_M8 | 82.0% | 16.9 | |||
G176_M8_sorted | 95.7% | 16.2 | |||
G176_M8_statistics_for_all_accepted_reads | 37.6 | ||||
G176_M8_statistics_for_primary_reads | 35.3 | ||||
G176_M8_statistics_for_primary_unique_reads | 33.9 | ||||
G176_M9_G176_M9 | 76.4% | 9.5 | |||
G176_M9_sorted | 95.8% | 9.1 | |||
G176_M9_statistics_for_all_accepted_reads | 21.6 | ||||
G176_M9_statistics_for_primary_reads | 19.8 | ||||
G176_M9_statistics_for_primary_unique_reads | 18.9 |
RSeQC
RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.
Infer experiment
Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).
featureCounts
Subread featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations.
Samtools
Samtools is a suite of programs for interacting with high-throughput sequencing data.
Samtools Flagstat
This module parses the output from samtools flagstat
. All numbers in millions.