A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.
Report generated on 2024-03-11, 13:14 based on data in:
/projectnb/wax-es/zzhang/zz_G221/SAMPLES
/projectnb/wax-es/zzhang/zz_G221/Scripts/03_FASTQC
/projectnb/wax-es/zzhang/zz_G221/Scripts/06_CollectMetrics
General Statistics
Showing 13/13 rows and 3/4 columns.Sample Name | M Reads Mapped | % Assigned | M Assigned |
---|---|---|---|
G221_M69_sorted | 93.3% | 5.8 | |
G221_M70_sorted | 93.9% | 6.7 | |
G221_M71_sorted | 93.6% | 7.4 | |
G221_M72_sorted | 94.7% | 5.7 | |
G221_M73_sorted | 93.8% | 17.4 | |
G221_M74_sorted | 92.8% | 11.8 | |
G221_M75_sorted | 93.8% | 17.2 | |
G221_M76_sorted | 93.5% | 6.7 | |
G221_M77_sorted | 94.3% | 6.4 | |
G221_M78_sorted | 94.3% | 7.1 | |
G221_M79_sorted | 94.4% | 6.7 | |
G221_M80_sorted | 94.7% | 6.7 | |
statistics_for_primary_unique_reads | 14.1 |
RSeQC
RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.
Infer experiment
Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).
featureCounts
Subread featureCounts is a highly efficient general-purpose read summarization program that counts mapped reads for genomic features such as genes, exons, promoter, gene bodies, genomic bins and chromosomal locations.
Samtools
Samtools is a suite of programs for interacting with high-throughput sequencing data.
Samtools Flagstat
This module parses the output from samtools flagstat
. All numbers in millions.