FastQCFastQC Report
Mon 6 Mar 2017
G151-M1-Index-8_ACTTGA-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M1-Index-8_ACTTGA-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27150749
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16000.021.58254443
CTCGTAT15850.020.95572342
TCTCGTA15100.020.68517341
CGTATGC16800.020.42403244
CGTCTGA17900.019.2935816
AATCTCG18750.016.89314839
GATCGGA20750.016.1241421
ATCGGAA20750.016.1126462
ATCTCGT20250.015.9674140
ACGTCTG22600.015.47577815
ACACGTC24000.014.208513
TCGGAAG25150.013.6402253
CGGAAGA28800.011.9882934
CACGTCT38850.09.68272714
GCACACG39300.09.3486411
CACACGT43450.08.35448212
GTCACAC69450.05.669852329
TTGAATC71150.05.318325536
GAACTCC72700.05.177049621
Produced by FastQC (version 0.11.2)