FastQCFastQC Report
Mon 6 Mar 2017
G151-M10-Index-21_GTTTCG-_BCALRAANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M10-Index-21_GTTTCG-_BCALRAANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24402850
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4300.026.09808512
GCGTCGT5000.022.00431311
AGCGTCG5350.021.3872410
GTCGCCG4550.018.37651344
GATCGGA9600.011.9181231
GAGCGTC9850.011.8398119
GATCTCG10300.010.6779434
GTCGTGT11100.010.50654213
TCGTGTA11650.09.82164714
ATCGGAA11900.09.7995182
CGTGTAG11950.09.75913515
TGGTCGC9300.08.98927842
GGTCGCC12050.08.39917843
AGAGCGT15450.08.118038
GTGGTCG12000.07.698921741
CGGTGGT15300.07.044317239
AAGAGCG16700.06.71972857
TCGGTGG15000.06.598778238
TCGGAAG17650.06.35770463
ATCTCGG15700.06.304732335
Produced by FastQC (version 0.11.2)