FastQCFastQC Report
Mon 6 Mar 2017
G151-M11-Index-22_CGTACG-_BCALRAANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M11-Index-22_CGTACG-_BCALRAANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28567705
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG6650.027.46395712
GTCGCCG5500.024.80323444
AGCGTCG7600.024.32040610
GCGTCGT7650.023.87389811
GTCGTGT13550.014.45297813
GAGCGTC13950.013.56539
GATCGGA13400.013.4642531
TCGTGTA15050.013.1586914
TGGTCGC10600.012.86807142
CGTGTAG16800.011.78782815
ATCGGAA16000.011.688862
GGTCGCC12500.011.26509443
AGAGCGT18600.011.1202748
GATCTCG14650.010.81061134
GTGGTCG13350.09.88636841
AAGAGCG20150.09.8279457
TCGGAAG22350.08.9586313
TCGGTGG20500.08.7984238
TCTCGGT19900.08.40066436
ATCTCGG19400.08.27702335
Produced by FastQC (version 0.11.2)