FastQCFastQC Report
Mon 6 Mar 2017
G151-M4-Index-11_GGCTAC-_BCALRAANXX_L001_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M4-Index-11_GGCTAC-_BCALRAANXX_L001_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27126881
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA12300.019.49568732
CGGCTAC12700.018.70873533
CTCGTAT13100.018.30193942
TCGTATG13500.017.10688843
TCTCGTA12950.016.98528941
CGTATGC14400.016.03788644
CGTCTGA16400.015.15658416
TCACGGC18750.013.49286530
ATCGGAA19500.012.7458072
GATCGGA18800.012.6441311
ACACGTC20000.012.32002613
ACGTCTG21500.012.2774215
CACGGCT22900.011.52821731
GTCACGG21700.011.45537929
ATCTCGT20150.010.91578240
AGTCACG23100.010.85612428
TCGGAAG23400.010.5252973
CATCTCG21900.09.94348439
CGGAAGA27600.08.9244
CACGTCT34000.08.08764114
Produced by FastQC (version 0.11.2)