FastQCFastQC Report
Mon 6 Mar 2017
G151-M6-Index-16_CCGTCC-_BCALRAANXX_L001_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M6-Index-16_CCGTCC-_BCALRAANXX_L001_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25801346
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG9450.029.1062212
GTCGCCG7550.027.97767644
GCGTCGT10150.026.88210111
AGCGTCG10300.025.84971410
GTCGTGT14900.018.01662813
CGTGTAG15650.017.85604115
GAGCGTC14950.017.8093629
TGGTCGC12150.017.3832542
TCGTGTA16300.016.73907914
GGTCGCC14100.015.91664743
GATCTCG15500.015.89464234
GTGGTCG13350.015.8184741
GATCGGA19300.014.2485631
AGAGCGT19600.013.9208728
ATCGGAA21250.013.0443982
AAGAGCG21550.012.7632447
ATCTCGG20450.012.69267635
TCTCGGT19050.012.58623836
TCGGTGG20600.011.63919438
TCGGAAG24750.011.1997363
Produced by FastQC (version 0.11.2)