FastQCFastQC Report
Mon 6 Mar 2017
G151-M7-Index-18_GTCCGC-_BCALRAANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M7-Index-18_GTCCGC-_BCALRAANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22375311
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC12100.036.53975733
ACGTCCG12600.034.9152732
TCCGCAC15450.028.47440135
GTCCGCA15800.027.84357334
TCTCGTA15250.026.82860643
CTCGTAT16850.025.45591744
CGTCTGA17900.024.45986616
GATCGGA18200.024.0720711
CCGCACA17800.023.9734736
ATCGGAA19250.022.8560852
ATCTCGT19700.021.10274342
CGCACAT20600.019.96731637
ACACGTC22150.019.9664413
ACGTCTG22950.019.26910615
TCGGAAG22950.019.2635063
AGTCACG25050.018.2662128
GTCACGT24950.018.2510929
CATCTCG23250.018.164341
CGGAAGA26650.016.4250244
CACGTCT33550.013.70575314
Produced by FastQC (version 0.11.2)