FastQCFastQC Report
Mon 6 Mar 2017
G151-M7-Index-18_GTCCGC-_BCALRAANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M7-Index-18_GTCCGC-_BCALRAANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22375311
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG11850.034.53908512
GCGTCGT12500.032.3910511
GTCGCCG9200.032.04887844
AGCGTCG12700.031.88095310
GTCGTGT17650.023.93716413
GAGCGTC17100.023.8063499
GATCGGA17800.023.6095051
TGGTCGC12200.023.26231442
CGTGTAG19200.021.88944615
GGTCGCC13700.021.8421243
GATCTCG16500.021.32953534
GTGGTCG13750.021.27711741
TCGTGTA20000.021.01452614
ATCGGAA20350.020.8673532
AGAGCGT21050.019.7570928
AAGAGCG21800.019.1780577
TCGGAAG24400.017.7646123
TCGGTGG19750.016.92844438
CGGAAGA26050.016.808714
TCTCGGT19150.016.76978536
Produced by FastQC (version 0.11.2)