FastQCFastQC Report
Mon 6 Mar 2017
G151-M8-Index-19_GTGAAA-_BCALRAANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M8-Index-19_GTGAAA-_BCALRAANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25619778
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT13150.020.24023644
TCTCGTA12450.019.43480343
AACGATC13300.018.52274938
CGTCTGA16700.017.52223616
GATCTCG14550.017.08275241
CGATCTC15150.016.11548640
TGAAACG18200.016.07439235
GATCGGA18350.015.596041
ACGATCT17100.014.66381439
GAAACGA19800.014.33093736
ATCGGAA20950.013.8606092
ACACGTC20400.013.69769213
ATCTCGT18300.013.58216342
ACGTCTG21250.013.45967315
GTCACGT22800.012.92874729
AAACGAT20150.012.66277137
AGTCACG23950.012.30809628
TCGGAAG23900.012.2396513
CGGAAGA27250.010.9779594
GCACACG32950.09.2150111
Produced by FastQC (version 0.11.2)