FastQCFastQC Report
Mon 6 Mar 2017
G151-M9-Index-20_GTGGCC-_BCALRAANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M9-Index-20_GTGGCC-_BCALRAANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25545545
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11450.016.90605744
TCTCGTA11400.016.01542343
GATCGGA12400.015.8007591
CGTCTGA14450.013.24625416
ATCGGAA15800.012.8095852
TATCTCG14700.012.41956541
ACACGTC17150.011.28967713
ACGTCTG18700.010.58859515
CGTGGCC19050.010.50769233
ATCTCGT17250.010.45627942
AGTCACG20550.010.38360128
GTCACGT19850.010.19568329
ACGTGGC21200.010.06456832
TCGGAAG20950.09.658933
CGGAAGA24650.08.3879144
CACGTCT28100.06.968249314
GCACACG32400.06.587030411
CACACGT32950.06.477080312
TCACGTG33700.06.201214330
CACGTGG39350.05.757701431
Produced by FastQC (version 0.11.2)