FastQCFastQC Report
Mon 6 Mar 2017
G151-M9-Index-20_GTGGCC-_BCALRAANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M9-Index-20_GTGGCC-_BCALRAANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25545545
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG6500.028.43629612
AGCGTCG7550.024.77297810
GTCGCCG6150.023.25541544
GCGTCGT7950.023.24980211
GAGCGTC12750.015.1872359
TGGTCGC10100.015.029592542
GATCGGA13400.014.2853231
GTCGTGT13600.014.07626613
GATCTCG11600.014.03229134
TCGTGTA14450.013.24824914
CGTGTAG15150.013.07161715
GTGGTCG12800.012.88899141
GGTCGCC11550.012.76311143
ATCGGAA15850.012.3548182
AGAGCGT17850.011.2177798
AAGAGCG18450.010.8528887
ATCTCGG16850.010.18232735
TCGGAAG21700.08.9229043
TCGGTGG19700.08.70909638
CGGAAGA25200.08.4696184
Produced by FastQC (version 0.11.2)