FastQCFastQC Report
Mon 6 Mar 2017
G151-M12-Index-23_GAGTGG-_BCALRAANXX_L002_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M12-Index-23_GAGTGG-_BCALRAANXX_L002_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26557590
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG9200.026.31030712
GTCGCCG7800.026.23493244
GCGTCGT10550.023.56936511
AGCGTCG10500.023.47185110
TGGTCGC11900.017.56309942
TCGTGTA16000.015.5410214
GATCTCG14150.015.38953934
GTGGTCG13850.015.24692341
GAGCGTC16900.015.1039279
CGTGTAG17000.015.01488315
GTCGTGT16700.014.889613
GGTCGCC14850.013.92745443
AGAGCGT22250.011.4720888
ATCTCGG19850.011.3027735
TCGGTGG22000.010.89799438
TCTCGGT22450.010.67960936
AAGAGCG25650.09.9512167
CGGTGGT22500.09.58027939
CTCGGTG26000.09.55983337
ATCGGAA27650.09.0716052
Produced by FastQC (version 0.11.2)