FastQCFastQC Report
Mon 6 Mar 2017
G151-M13-Index-25_ACTGAT-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M13-Index-25_ACTGAT-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25993596
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG6550.027.2114612
GCGTCGT7700.023.14750111
AGCGTCG8050.022.14108710
GTCGCCG6600.021.3359344
GTCGTGT12350.014.61002113
GAGCGTC13700.013.6522689
TGGTCGC10500.013.19952342
GTGGTCG12100.012.18032541
TCGTGTA15150.011.76453314
GATCTCG12950.011.7203934
GGTCGCC12500.011.08879643
GATCGGA15850.010.9658331
CGTGTAG16550.010.76926215
ATCGGAA17150.010.6477682
AGAGCGT18850.010.155638
TCGGTGG18100.09.35757338
AAGAGCG20100.09.3051887
ATCTCGG18600.08.51485635
CGGTGGT17350.08.49421139
TCGGAAG23150.08.2689363
Produced by FastQC (version 0.11.2)