FastQCFastQC Report
Mon 6 Mar 2017
G151-M14-Index-27_ATTCCT-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M14-Index-27_ATTCCT-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24489933
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG7800.027.36480512
AGCGTCG7700.026.86297610
GCGTCGT8250.025.87228411
GTCGCCG7750.021.86089144
GTCGTGT14350.015.48758913
TCGTGTA15000.014.81633914
TGGTCGC11500.014.72999342
GAGCGTC15800.014.2054719
GATCGGA15800.013.9246291
GTGGTCG14250.013.12107441
CGTGTAG17450.012.98822915
ATCGGAA17600.012.8755872
GATCTCG14900.012.400743534
AGAGCGT19450.011.65267858
GGTCGCC14800.011.29829743
AAGAGCG20950.010.7133027
ATCTCGG17800.010.38033435
TCTCGGT18250.010.24482436
TCGGTGG19800.010.22037138
TCGGAAG24300.09.3262713
Produced by FastQC (version 0.11.2)