FastQCFastQC Report
Mon 6 Mar 2017
G151-M15-Index-1_ATCACG-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M15-Index-1_ATCACG-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25046772
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9250.015.2479791
TCTCGTA9550.014.26129541
CTCGTAT9850.013.60395342
TCGTATG10200.013.56882743
CGATCTC12050.012.39685838
ATCGGAA12350.012.125692
CGTATGC11450.012.08847844
GATCTCG11900.011.62958639
ACGATCT13050.011.61640337
CGTCTGA14100.010.307075516
CACGATC14500.010.00130636
ACACGTC16200.09.37857513
ACGTCTG17000.09.19580215
TCACGAT15300.09.19148835
CATCACG17350.09.11889933
ATCTCGT15550.09.04104240
TCGGAAG17100.08.8857843
ATCACGA18250.07.826376434
CGGAAGA21950.07.32416344
CACGTCT28500.05.94892114
Produced by FastQC (version 0.11.2)