FastQCFastQC Report
Mon 6 Mar 2017
G151-M15-Index-1_ATCACG-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M15-Index-1_ATCACG-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25046772
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5000.027.72573912
AGCGTCG5900.023.49638710
GTCGCCG5100.022.43453644
GCGTCGT6100.022.36528211
GATCGGA10700.013.7760481
GAGCGTC10100.013.507619
GTCGTGT11800.013.05344413
TGGTCGC9600.012.14514542
GATCTCG12250.012.03091434
ATCGGAA12450.011.839682
TCGTGTA12200.011.72359714
GTGGTCG11550.011.04604541
GGTCGCC12350.09.97655843
CGTGTAG15150.09.73123115
AGAGCGT14900.09.4514688
AAGAGCG16250.09.2079317
TCGGAAG18600.08.635423
ATCTCGG16900.08.46021535
TCTCGGT17050.08.1277636
TCGGTGG17350.08.11397238
Produced by FastQC (version 0.11.2)