FastQCFastQC Report
Mon 6 Mar 2017
G151-M16-Index-2_CGATGT-_BCALRAANXX_L003_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M16-Index-2_CGATGT-_BCALRAANXX_L003_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25262654
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11400.020.42571842
CGTATGC11650.019.80181544
TCGTATG11900.019.56865743
CGATGTA12200.019.27241134
TCTCGTA12100.019.24406441
ACCGATG13100.017.78043732
CACCGAT13400.017.21838231
CGTCTGA14450.016.30522316
CCGATGT15100.015.57102833
GTCACCG15650.015.303287529
TATCTCG16550.013.93708239
ACACGTC17450.013.62819313
ACGTCTG18250.012.78869515
GATCGGA18500.012.5074791
ATCGGAA19750.011.9304732
ATCTCGT19250.011.7539940
TCACCGA22550.010.42599630
TCGGAAG24750.09.6982453
CGGAAGA27300.08.7119434
CACGTCT30750.08.09144414
Produced by FastQC (version 0.11.2)