FastQCFastQC Report
Mon 6 Mar 2017
G151-M18-Index-4_TGACCA-_BCALRAANXX_L003_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M18-Index-4_TGACCA-_BCALRAANXX_L003_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23853131
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG5650.023.75740612
GTCGCCG5600.021.61005644
GCGTCGT6350.021.48501411
AGCGTCG6450.021.15191310
TGGTCGC8750.013.57678242
GATCGGA10550.012.9293251
TCGTGTA11250.012.9092214
GAGCGTC11250.012.7136529
GTCGTGT11300.012.46282513
GATCTCG10650.012.39247834
CGTGTAG12200.012.26464415
GTGGTCG9550.012.20779341
ACGGTCG1300.003581945311.84462225
GGTCGCC11750.010.67335443
ATCGGAA13600.010.1915252
AGAGCGT14200.09.917338
AAGAGCG16250.08.8017237
ATCTCGG16400.08.58396735
TCTCGGT16950.08.04583736
TCGGTGG16700.07.902755738
Produced by FastQC (version 0.11.2)