FastQCFastQC Report
Mon 6 Mar 2017
G151-M19-Index-5_ACAGTG-_BCALRAANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M19-Index-5_ACAGTG-_BCALRAANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27213117
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8750.09.54422842
TCGTATG9950.08.61389543
TCTCGTA9300.08.50716441
CGTATGC10100.07.833069344
GATCTCG13150.06.85213339
GATCGGA11450.06.54035571
CGTCTGA14400.05.80800316
ACACGTC16500.05.73568713
ATCGGAA14200.05.734982
ATCTCGT16700.05.65870740
ACGTCTG16800.05.371281615
Produced by FastQC (version 0.11.2)