FastQCFastQC Report
Mon 6 Mar 2017
G151-M19-Index-5_ACAGTG-_BCALRAANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M19-Index-5_ACAGTG-_BCALRAANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27213117
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG4450.016.8129512
GCGTCGT4800.015.12853711
GTCGCCG4250.013.97757944
AGCGTCG5200.013.96480310
GTCGTGT11050.08.1648213
TCGTGTA11350.07.561252614
GAGCGTC11200.07.465779
TGGTCGC9000.07.33312142
GATCGGA11700.06.9574721
ATCGGAA13450.06.54322242
CGTGTAG14200.06.198518315
GATCTCG10956.548362E-116.0263834
GTGGTCG11703.6925485E-105.64031241
AGAGCGT14801.8189894E-125.5010238
Produced by FastQC (version 0.11.2)