FastQCFastQC Report
Mon 6 Mar 2017
G151-M20-Index-6_GCCAAT-_BCALRAANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M20-Index-6_GCCAAT-_BCALRAANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24567347
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG9600.012.82001243
CGTATGC10150.012.77474744
CGCCAAT10050.012.03036333
CTCGTAT10100.011.96812842
TCTCGTA9700.011.78193241
TATCTCG12350.010.67757739
ACGCCAA12400.010.10497632
GTCACGC13000.09.29931729
CGTCTGA13500.09.29261916
GATCGGA13100.08.7431391
ATCGGAA14450.08.0724282
ACACGTC16000.07.56544413
ACGTCTG17050.07.357776615
ATCTCGT16200.07.325944440
TCACGCC17500.07.28522430
AGTCACG19000.07.056802728
TCGGAAG18700.06.7086443
CACGCCA18950.06.38012931
CGGAAGA23100.05.7177924
TCCGATC6000.00336704945.1354473
Produced by FastQC (version 0.11.2)