FastQCFastQC Report
Mon 6 Mar 2017
G151-M22-Index-8_ACTTGA-_BCALRAANXX_L004_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M22-Index-8_ACTTGA-_BCALRAANXX_L004_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26520906
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8850.010.18156642
TCTCGTA8850.09.43657341
TCGTATG9400.09.35180643
CGTATGC10850.08.3045644
CGTCTGA13300.06.94986616
AATCTCG11850.06.862156439
ACGTCTG16600.05.833443615
ATCTCGT14250.05.706382340
GATCGGA14750.05.6744411
ACACGTC16850.05.4856413
ATCGGAA17150.05.26188232
Produced by FastQC (version 0.11.2)