FastQCFastQC Report
Mon 6 Mar 2017
G151-M23-Index-9_GATCAG-_BCALRAANXX_L004_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M23-Index-9_GATCAG-_BCALRAANXX_L004_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28079344
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG11450.032.4786412
GCGTCGT12550.030.33320211
AGCGTCG13250.029.06284110
GTCGCCG12400.022.8884644
GATCGGA17850.021.5692211
TCGTGTA18550.019.69159114
ATCGGAA20200.019.604992
GAGCGTC19950.019.4118219
TGGTCGC14550.019.35324342
GTCGTGT20300.018.64442813
AGAGCGT22350.017.81938
CGTGTAG21150.017.58259215
GATCTCG19200.017.07043634
GTGGTCG18100.015.9208541
AAGAGCG25750.015.8081757
ATCTCGG23400.014.6643835
TCGGAAG26500.014.6128433
TCGGTGG26150.012.70147138
GGTCGCC22700.012.69638443
TCTCGGT26750.012.33452536
Produced by FastQC (version 0.11.2)