FastQCFastQC Report
Mon 6 Mar 2017
G151-M26-Index-15_ATGTCA-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M26-Index-15_ATGTCA-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27659597
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT28650.032.6256244
TCTCGTA28450.031.07795343
CGTCTGA33600.029.14326916
GATCGGA34900.027.6286871
AATCTCG31850.027.62265441
ACGTCTG38800.025.52121715
ATCGGAA38450.025.228472
ACACGTC38600.025.1403413
ATCTCGT35900.024.81246842
TCGGAAG42000.023.4604173
CGGAAGA44300.022.0454654
CACGTCT48350.020.34378214
CACACGT54150.018.36791412
GCACACG55600.018.20511611
GAACTCC92850.011.02266921
ACATGTC93750.010.86227132
GTCACAT101950.010.22570929
CAGTCAC108350.09.72440627
TCTGAAC112700.09.60613118
GAATCTC99500.09.52728440
Produced by FastQC (version 0.11.2)