FastQCFastQC Report
Mon 6 Mar 2017
G151-M27-Index-16_CCGTCC-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M27-Index-16_CCGTCC-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27169888
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG27400.031.54688343
CGTATGC27950.031.39727644
CTCGTAT27400.031.22579642
TCTCGTA26600.030.84233341
ACCCGTC30500.028.98960332
CGTCTGA32150.027.65165716
CCGTCCA31700.027.1977234
TCACCCG33500.026.32767130
CGTCCAT31900.026.13098535
CACCCGT34050.026.09638431
ACACGTC35800.025.01705213
ATCTCGT33400.024.76061640
ACGTCTG37350.024.09669515
CATCTCG35000.023.6913739
ATCGGAA39450.023.027092
GATCGGA39700.022.7901121
CCCGTCC39400.022.1619933
TCGGAAG45000.020.2837583
CGGAAGA45850.019.8130954
CACGTCT48350.018.61450814
Produced by FastQC (version 0.11.2)