FastQCFastQC Report
Mon 6 Mar 2017
G151-M27-Index-16_CCGTCC-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M27-Index-16_CCGTCC-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27169888
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG22750.037.14204812
GCGTCGT23350.036.37632811
AGCGTCG23600.035.71125810
GTCGCCG16850.035.6462644
GTCGTGT28850.030.05090913
CGTGTAG30750.028.40820515
GAGCGTC30000.028.0927039
GATCTCG24900.027.73897734
TCGTGTA31150.027.6199414
TGGTCGC21500.026.80766142
GGTCGCC23950.025.16992443
GTGGTCG24100.024.64302841
AGAGCGT34600.024.6122258
ATCTCGG30550.023.32819635
AAGAGCG37600.022.824057
GATCGGA37500.022.8228761
ATCGGAA37850.022.7283842
TCGGTGG31900.021.85775238
TCTCGGT32050.020.72635836
TCGGAAG43800.019.5909423
Produced by FastQC (version 0.11.2)