FastQCFastQC Report
Mon 6 Mar 2017
G151-M28-Index-18_GTCCGC-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M28-Index-18_GTCCGC-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24562165
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC23750.037.04342733
ACGTCCG23650.037.0142132
CTCGTAT27400.032.02671444
TCCGCAC28100.031.62158635
GTCCGCA27700.031.60161434
TCTCGTA26650.030.94859143
CCGCACA32150.027.91173736
CGTCTGA33050.027.8968716
GATCGGA32800.027.1797451
ATCGGAA34100.026.0594752
CGCACAT32600.026.04266437
ACACGTC35100.025.7036613
GTCACGT36050.025.13635629
ACGTCTG36750.025.02863315
ATCTCGT33100.024.85142542
AGTCACG37800.023.97317328
CATCTCG34550.023.93621441
TCGGAAG38650.023.2741853
CGGAAGA41950.021.6553694
CACGTCT48200.019.40260714
Produced by FastQC (version 0.11.2)