FastQCFastQC Report
Mon 6 Mar 2017
G151-M28-Index-18_GTCCGC-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M28-Index-18_GTCCGC-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24562165
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG22150.038.84333812
AGCGTCG23050.037.04036310
GCGTCGT23550.036.53425611
GTCGCCG17250.034.9481144
GTCGTGT27900.030.91623313
TCGTGTA29000.029.81936514
GAGCGTC29250.029.6404489
TGGTCGC20750.029.47294442
CGTGTAG30250.028.73228815
GATCTCG24800.027.4961234
GATCGGA32850.026.9916381
GTGGTCG24900.025.79487641
ATCGGAA35100.025.6372972
AGAGCGT34250.025.6345588
GGTCGCC24850.024.52447543
AAGAGCG37050.024.1129557
ATCTCGG29350.023.83227235
TCGGAAG40200.022.385583
TCTCGGT30200.022.28724936
TCGGTGG32950.021.76191338
Produced by FastQC (version 0.11.2)