FastQCFastQC Report
Mon 6 Mar 2017
G151-M29-Index-19_GTGAAA-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M29-Index-19_GTGAAA-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27759577
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG19750.037.43620312
GCGTCGT20900.035.48166311
AGCGTCG21250.035.00081310
GTCGCCG16400.034.34417344
GTCGTGT26650.027.74302513
TCGTGTA26850.027.7002314
GAGCGTC27100.027.4451879
TGGTCGC20150.026.420442
CGTGTAG29200.025.54605915
GTGGTCG22850.024.93271641
GATCTCG25500.024.32602734
GATCGGA32650.023.315021
ATCGGAA32650.023.247972
AGAGCGT32450.022.9880968
AAGAGCG35300.021.692957
GGTCGCC26250.021.53993243
ATCTCGG30350.021.1626135
TCTCGGT30500.020.55340836
TCGGTGG32600.020.44386938
TCGGAAG40000.019.0867123
Produced by FastQC (version 0.11.2)