FastQCFastQC Report
Mon 6 Mar 2017
G151-M30-Index-20_GTGGCC-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M30-Index-20_GTGGCC-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26055024
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA25500.030.18893443
GATCGGA27750.030.0634541
CTCGTAT27250.029.5410144
CGTCTGA29750.027.81143416
ATCGGAA30400.027.2772792
TATCTCG29600.025.71062741
CGTGGCC32700.025.56009333
ACGTCTG33300.025.24318515
ACACGTC33100.024.99682613
GTCACGT33650.024.64210929
ACGTGGC34000.024.58279632
AGTCACG34650.023.86759628
ATCTCGT32850.023.56824342
TCGGAAG35500.023.2956713
CGGAAGA40650.020.0756664
CACGTCT43950.019.07616614
TCACGTG46300.018.19460930
GCACACG49750.016.98467811
CACACGT50100.016.73448212
CACGTGG53350.015.87277531
Produced by FastQC (version 0.11.2)