FastQCFastQC Report
Mon 6 Mar 2017
G151-M30-Index-20_GTGGCC-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M30-Index-20_GTGGCC-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26055024
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG15950.038.20993444
AGCGTCG21050.037.94752510
GCGTCGT21250.037.3832611
CGTCGTG21350.037.1049512
GAGCGTC25650.031.056279
GATCGGA26950.029.4708271
GTCGTGT28050.028.94736713
TGGTCGC21000.028.17913442
GATCTCG23900.028.07102434
TCGTGTA28800.027.964114
ATCGGAA29200.027.7278082
GTGGTCG23000.026.68211241
CGTGTAG30950.026.2345115
GGTCGCC23750.026.03069343
AGAGCGT32350.024.9643278
TCGGTGG29700.023.92098438
AAGAGCG34000.023.7526867
ATCTCGG29950.023.1344535
TCGGAAG35950.022.82843
TCTCGGT30100.022.50736836
Produced by FastQC (version 0.11.2)