FastQCFastQC Report
Mon 6 Mar 2017
G151-M31-Index-21_GTTTCG-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M31-Index-21_GTTTCG-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26102330
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG18400.037.4336912
GCGTCGT19550.035.3444711
AGCGTCG20200.034.53396610
GTCGCCG15050.033.6236644
GTCGTGT23900.029.2787713
TCGTGTA25850.026.8142814
CGTGTAG26150.026.7585415
GAGCGTC26350.026.6407349
TGGTCGC19050.025.7513742
GATCTCG23150.024.22942234
GATCGGA29150.023.699761
GGTCGCC21700.023.11561843
ATCGGAA30050.022.9899062
AGAGCGT30850.022.7547268
GTGGTCG22900.022.47648441
AAGAGCG32550.021.5662677
ATCTCGG27900.020.89214335
TCGGAAG33100.020.7390793
TCTCGGT28800.019.55170636
CGGAAGA37800.018.3357334
Produced by FastQC (version 0.11.2)