FastQCFastQC Report
Mon 6 Mar 2017
G151-M32-Index-22_CGTACG-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M32-Index-22_CGTACG-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26067760
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG21500.037.15226710
GCGTCGT21600.036.98026711
GTCGCCG15650.036.9741144
CGTCGTG21650.036.69144412
GTCGTGT26850.029.83035513
GAGCGTC27200.029.6902249
TCGTGTA27500.028.96513414
GATCGGA27750.028.8586731
TGGTCGC20550.028.15406242
CGTGTAG29150.027.55184215
ATCGGAA30650.026.4162482
GATCTCG25300.026.08337234
GTGGTCG22950.025.97399541
GGTCGCC22600.025.89481743
AGAGCGT32150.025.2557858
AAGAGCG33850.024.0523157
TCTCGGT29150.022.56237436
TCGGTGG31750.022.03077338
ATCTCGG30350.021.96031635
TCGGAAG36950.021.9128063
Produced by FastQC (version 0.11.2)