FastQCFastQC Report
Mon 6 Mar 2017
G151-M33-Index-23_GAGTGG-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M33-Index-23_GAGTGG-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28146953
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG23850.040.0436712
GTCGCCG20300.037.06768844
GCGTCGT26050.036.83081411
AGCGTCG26750.035.7847510
GTCGTGT30250.031.49803713
GAGCGTC32400.029.884059
TGGTCGC26050.029.0495742
TCGTGTA33300.028.41458714
GATCTCG28450.028.2203634
GTGGTCG27500.028.15471641
CGTGTAG35150.027.60731715
GGTCGCC28100.027.48166543
ATCGGAA38250.025.481072
GATCGGA38200.025.45661
AGAGCGT38300.025.4528688
AAGAGCG40450.024.6438837
ATCTCGG33450.024.52748935
TCGGTGG35200.024.24484638
TCTCGGT35400.022.67911736
TCGGAAG44800.021.9527783
Produced by FastQC (version 0.11.2)