FastQCFastQC Report
Mon 6 Mar 2017
G151-M34-Index-25_ACTGAT-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M34-Index-25_ACTGAT-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26753925
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT273180.10210838222802822TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35150.036.85429444
TCTCGTA36250.034.52328543
TATCTCG39100.032.11992341
CGTCTGA42750.031.24493416
ACACGTC44850.029.83102613
ACGTCTG44950.029.56889515
ATCTCGT43150.029.15567242
GATCGGA46700.028.4703871
ATCGGAA48100.027.7111192
TCGGAAG53800.024.8145333
CACGTCT55900.023.97360614
CGGAAGA57600.023.0655864
GCACACG63050.021.56861911
CACACGT65650.021.01647412
GTCACAC94050.014.56930129
GAACTCC101650.013.5758721
ACTGATA103000.013.45272834
GATATAT105250.012.684792537
CAGTCAC113000.012.24390227
ATATCTC107950.012.18410640
Produced by FastQC (version 0.11.2)