FastQCFastQC Report
Mon 6 Mar 2017
G151-M34-Index-25_ACTGAT-_BCALRAANXX_L005_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M34-Index-25_ACTGAT-_BCALRAANXX_L005_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26753925
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG32150.040.38189712
GCGTCGT32850.039.65551811
AGCGTCG32850.039.3205910
GTCGCCG26100.038.52485744
GAGCGTC38750.033.6743059
GTCGTGT38500.033.60656713
GATCTCG33050.032.7453334
TGGTCGC30350.031.82032442
CGTGTAG41050.031.6259215
TCGTGTA40850.031.56547214
GTGGTCG33350.030.207941
GGTCGCC33800.029.87733543
AGAGCGT44600.029.356068
AAGAGCG46850.028.0401047
GATCGGA46450.027.8986741
ATCTCGG41250.026.87535535
ATCGGAA48200.026.7492162
TCGGTGG42200.026.68683238
TCTCGGT40000.026.67004236
CGGTGGT39350.026.10369139
Produced by FastQC (version 0.11.2)