FastQCFastQC Report
Mon 6 Mar 2017
G151-M35-Index-27_ATTCCT-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M35-Index-27_ATTCCT-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28457132
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT23650.030.78150644
TCTCGTA23600.029.63568743
CGTCTGA28850.026.7726716
TATCTCG26900.026.49125541
GATCGGA29600.025.2840521
ACGTCTG31600.024.44281415
ATCGGAA31100.023.9749812
ATCTCGT31250.022.9439542
ACACGTC34800.021.94226313
TCGGAAG38250.019.9524213
CGGAAGA41150.018.7087124
CACGTCT44550.017.83161514
GCACACG50550.015.67129511
CACACGT51850.015.23620612
GAACTCC91450.08.85694321
GTCACAT98400.08.560616529
CTTTATC102700.07.773998338
ACATTCC106600.07.634023732
TCTGAAC111500.07.44013618
GTCTGAA116100.07.410694617
Produced by FastQC (version 0.11.2)