FastQCFastQC Report
Mon 6 Mar 2017
G151-M36-Index-1_ATCACG-_BCALRAANXX_L005_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG151-M36-Index-1_ATCACG-_BCALRAANXX_L005_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29172999
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18100.025.51849743
TCTCGTA17750.024.03936441
CTCGTAT19750.022.6070642
CGTATGC20650.022.57966844
GATCGGA21800.021.5076031
ATCGGAA23750.020.0043242
CGATCTC21550.019.90209438
GATCTCG22600.018.97759839
TCACGAT24150.018.3061535
CGTCTGA26400.018.25411816
CACGATC24100.018.1615736
ACGATCT24050.017.83335937
ACACGTC28500.016.98631713
ATCTCGT25950.016.9516640
ATCACGA27400.016.85728334
CATCACG29400.016.30927733
ACGTCTG29750.016.124715
TCGGAAG33700.014.2929753
CGGAAGA35850.013.2529024
CACGTCT40800.011.86546214
Produced by FastQC (version 0.11.2)