FastQCFastQC Report
Wed 28 Mar 2018
RNAseq_NIPBL_Rep4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameRNAseq_NIPBL_Rep4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62824732
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2949210.4694345532584206No Hit
CCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTC2415590.3844966660582014No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2388540.3801910368674553No Hit
CACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCA1692290.2693668474383623No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1366190.2174605376748762TruSeq Adapter, Index 11 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTG1345540.21417361557547113No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1225920.1951333433463751No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1149160.1829152251696036No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1133320.18039392511853453No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1080110.17192433069193197No Hit
ACCACAAATTATGCAGTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGT872830.13893095477112422No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG829550.13204194806593048No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG801830.1276296729765596No Hit
GTCGAGTTTCCCGCATTTGGGGAAATCGCAGGGGTCAGCACATCCGGAGT786350.1251656751993785No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG771100.12273828720829243No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC756900.12047803084937951No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG660270.1050971454999601No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC634820.10104619308204928No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA630930.10042701017809355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA163450.039.603881
CGGCTAC172000.037.29992733
ACGGCTA172400.037.26540832
ATCGGAA180200.035.8973352
CGTCTGA188450.034.5169316
ACGTCTG195500.033.33968715
ATCTCGT194100.033.0760640
CGTATGC196550.032.7603744
TCGGAAG219400.029.9247683
AGTCACG217850.029.71174228
CGGAAGA236200.028.204274
CATCTCG229700.028.06474139
TCGTATG241200.026.69617543
TCACGGC252250.025.5121930
GCACACG258450.025.46605511
CACGGCT257800.025.25348531
GCTACAT264050.024.56400535
TCTCGTA273000.023.62124441
CTACATC277850.023.39928236
ACACGTC288250.023.00120413
Produced by FastQC (version 0.11.2)